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目的用正交设计法优化培养基中美拉德反应的条件,以提高猪苓菌核产量。方法以果糖和精氨酸作为美拉德反应的原料,经过调节pH后在一定温度下独立灭菌一定时间,之后与含蛋白胨和无机盐的培养基混合均匀并用于诱导猪苓菌核。以菌核干重为指标,对灭菌前pH值,果糖与精氨酸浓度比,灭菌时间(min),灭菌温度(℃)4个因素进行了L9(34)正交实验。结果极差分析结合方差分析表明,灭菌前pH值对菌核产量具有显著影响。优化后反应条件为:灭菌前pH为10;浓度比为200∶1;灭菌时间为80 min;灭菌温度为121℃。菌核产率提高了75%,菌核干重提高到每皿(0.225±0.16)g。结论相比优化前菌核产率为30%～50%,菌核干重为每皿0.1～0.15 g,新的反应条件显著提高了菌核产量。
Objective To optimize the Maillard reaction conditions in culture medium by orthogonal design to improve the production of Polyporus umbellatus. Methods Fructose and arginine were used as raw materials for the Maillard reaction. After the pH was adjusted, they were sterilized at a certain temperature for a certain period of time. After that, fructose and arginine were mixed well with the medium containing peptone and inorganic salts and used to induce E. coli. Taking the dry weight of sclerotia as an index, orthogonal test of L9 (34) was carried out on four factors: pH value before sterilization, concentration ratio of fructose to arginine, sterilization time (min) and sterilization temperature (℃) The results of range analysis combined with analysis of variance showed that the pH value before sterilization had a significant effect on the production of sclerotia. The optimized reaction conditions were as follows: the pH value before sterilization was 10; the concentration ratio was 200: 1; the sterilization time was 80 min; the sterilization temperature was 121 ℃. The yield of sclerotia increased by 75% and the dry weight of sclerotia increased to 0.225 ± 0.16 g per dish. Conclusion Compared to the pre-optimization sclerotia yield of 30% to 50%, sclerotia dry weight 0.1 ~ 0.15 g per dish, the new reaction conditions significantly increased sclerotia yield.