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以可再生的β-蒎烯衍生物诺蒎酮为原料,设计合成了一种新型的诺蒎酮基喹唑啉-2-胺铜离子荧光探针N-苄基-4-(4-(二乙氨基)苯基)-7,7-二甲基-5,6,7,8-四氢-6,8-桥亚甲基喹唑啉-2-胺(BNQ).探针BNQ在PBS/THF(V/V=8/2,10mmol·L-1,pH=7.4)溶液中对Cu2+表现出高度选择性的荧光猝灭效应,并能在较宽pH范围(4～10)内有效识别Cu2+.同时,荧光滴定实验结果表明,探针BNQ对Cu2+具有良好的灵敏度,其检测限为0.09 μmol·L-1,远低于世界卫生组织(WHO)规定在饮用水中Cu2+的最高含量(＜30 μmol·L-1).而且,采用高分辨质谱以及密度泛函理论计算确定了探针BNQ与Cu2+的络合作用机理.探针BNQ还可以对不同水环境中的微摩尔级Cu2+进行快速检测.生物成像实验发现,探针BNQ还可检测斑马鱼体内的Cu2+,并呈现出良好的荧光成像效果.“,”In this study,a novel nopinone-based quinazolin-2-amine fluorescent probe N-benzyl-4-(4-(diethylamino)phenyl)-7,7-dimethyl-5,6,7,8-tetrahydro-6,8-methanoquinazolin-2-amine (BNQ) was synthesized from renewable β-pinene derivative nopinone.The probe BNQ exhibited a high selective fluorescence quenching response toward Cu2+ in PBS/THF (V/V=8/2,10 mmol·L-1,pH=7.4) solution,and could effectively recognize Cu2+ within a wide pH range (4～ 10).Meanwhile,the fluorescent titration experiment showed that the probe BNQ was high sensitive for detecting Cu2+,and its detection limit was found to be 0.09 μmol·L-1,which was much lower than that of stated by World Health Organization (WHO) in drinking water (＜30 μmol·L-1).Furthermore,the coordination mechanism of probe BNQ with Cu2+ was confirmed by high resolution mass spectrum (HRMS) and density functional theory (DFT) calculations.The probe BNQ could detect micromolar Cu2+ in different environmental water samples.The results of bio-imaging experiment demonstrated that the probe BNQ was also successfully used for monitoring Cu2+ in living zebrafish,and showed an excellent fluorescence imaging performance.