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目的:观察莪术醇干预后,体外培养A549细胞凋亡诱导因子(apoptosis induce factor,AIF)和聚ADP核糖聚合酶[poly(ADP-ribose)polymerase,PARP]的表达情况,探讨莪术醇对非半胱天冬酶(caspase)依赖细胞凋亡的分子机制。方法:以不同质量浓度的莪术醇(12.5,25,50,100 mg.L-1)及阴性组处理肺癌细胞,药物作用48 h后提取各组蛋白;采用Westernblotting法分别检测各组AIF,PARP,caspase-3蛋白表达,并进行统计学分析。结果:经莪术醇处理后,阴性组的A549细胞的PARP相对蛋白表达量为0.545 3,AIF的相对蛋白表达量为0.358 6,而100 mg.L-1加药组的相对蛋白表达量分别为1.661 3和2.212 4,蛋白表达均随药物浓度的增加而增多,药物组与阴性组相比差异极显著(P<0.01),而caspase-3的前体(32×103)表达受药物影响较小,其激活体(17×103)基本不表达。结论:莪术醇可能主要是通过上调PARP蛋白的表达,进而将AIF转位到细胞核而引起凋亡的非依赖caspase途径来介导肺癌A549细胞的凋亡。
OBJECTIVE: To observe the expression of apoptosis inducing factor (AIF) and poly ADP-ribose polymerase (PARP) in A549 cells after curcumol intervention, and to explore the effect of curcumol on non- The molecular mechanism of caspase-dependent apoptosis. Methods: Lung cancer cells were treated with curcumol at various concentrations (12.5, 25, 50, 100 mg.L-1) and negative group for 48 h. Western blotting was used to detect the expression of AIF, PARP and caspase -3 protein expression, and statistical analysis. Results: After treatment with Curcumol, the expression of PARP protein in negative group was 0.545 3 and that of AIF was 0.358 6 in A549 cells, while the relative protein expression of A549 cells in 100 mg.L-1 group was 1.661 3 and 2.212 4. The protein expression increased with the increase of drug concentration, the difference between the drug group and the negative group was extremely significant (P <0.01), while the expression of the precursor of caspase-3 (32 × 103) Small, its activator (17 × 103) basically not expressed. Conclusion: Curcumol may induce the apoptosis of lung cancer A549 cells mainly by up-regulating the expression of PARP protein and then translocating AIF to the nucleus to cause apoptosis-independent caspase pathway.