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人体细胞体外恶性转化系统可望成为检测可疑致癌物和研究癌变原理的理想途径,故受到广泛重视。但迄今绝大部分工作仍属探索性质,尚未见将其用于实际检测与特定肿瘤相关的可疑化学致癌物质的报道。我们首次进行了这种尝试并获成功。 用河南林县食管癌高发区居民食物中存在的一种新亚硝胺—甲基丁基甲基丙酮基亚硝胺(MAMBNA)和二乙基亚硝胺(NDEA)分别处理离体培养的早代流产胎儿肺成纤维细胞。MAMBNA所用剂量为500、1000或1500微克/毫升,NDEA为300、900或1500微克。 处理的同时加入混合功能氧化
The malignant transformation system of human cells in vitro is expected to become an ideal way to detect suspected carcinogens and to study the principle of canceration, so it has received extensive attention. However, most of the work so far is still of an exploratory nature and has not yet been reported for the actual detection of suspected chemical carcinogens associated with specific tumors. We made this attempt for the first time and succeeded. A new nitrosamine-methyl butyl methyl acetonyl nitrosamine (MAMBNA) and diethylnitrosamine (NDEA) existed in foods from residents of high incidence areas of esophageal cancer in Linxian County of Henan Province were treated with early culture in vitro Aborted fetal lung fibroblasts. MAMBNA was used at doses of 500, 1000 or 1500 μg/ml and NDEA was at 300, 900 or 1500 μg. Add mixed function oxidation while processing