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用12天35℃初步适应培育出一株在KMB17细胞稳定传代、感染性滴度高、增殖周期短的HAV适应株LU8株,其感染性滴度为108.33TCID50/ml,抗原滴度为1:1024,一步生长曲线试验和免疫电镜观察结果表明12天为该株病毒增殖高峰。LUB株和H2减毒株HAV经纯化和灭活后接种豚鼠,第一次接种后4周血清HAV抗体均阳转,第二次接种后滴度为1:13.5和1:4.8(GMT),第三次接种后滴度增至1:16和1:8。提示LUB株抗原性较H2株强。
A strain of HAV strain LU8 strain that had stable passage in KMB17 cells with high titer of infection and short cycle of proliferation was cultivated with 12 days at 35 ℃. The infectious titer was 108.33 TCID50 / ml and the titer of antigen was 1 : 1024, one-step growth curve test and immunoelectron microscopy showed that the 12-day peak of the virus proliferation. The LAV strain and H2 attenuated strain HAV were inoculated with guinea pigs after purification and inactivation. Serum HAV antibody was positive at 4 weeks after the first inoculation, and the titer was 1: 13.5 and 1: 4.8 after the second inoculation (GMT), the titers increased to 1:16 and 1: 8 after the third inoculation. Tip LUB strain antigenicity stronger than the H2 strain.