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目的研究紫草组分的体外抗肿瘤作用及其作用机制。方法用MTT法、流式细胞仪,观察1.25～10.00μg.mL-1紫草组分对B16F10及HepG2细胞作用24 h的生长抑制作用及凋亡诱导作用;用激光共聚焦显微镜,观察药物的细胞分布;用Western-blot法检测细胞凋亡相关蛋白分子的变化。结果紫草组分能够抑制HepG2和B16F10细胞的增殖并诱导其早期凋亡,并呈浓度与时间依赖性。药物主要分布于细胞质中,在给药后30 min,给药组的p-JNK与p-p38分子明显增加。结论紫草组分可抑制HepG 2和B16F10细胞的增殖,并诱导细胞凋亡。
Objective To study the anti-tumor effect and its mechanism of comfrey component in vitro. Methods MTT assay and flow cytometry were used to observe the growth inhibition and apoptosis induction of B16F10 and HepG2 cells treated with 1.25 ~ 10.00μg.mL-1 Lithospermum components for 24 h. The expression of the drug was observed by laser confocal microscopy Cell distribution; Western blot analysis of apoptosis-related protein molecules. Results Lithospermum components could inhibit the proliferation of HepG2 and B16F10 cells and induce their early apoptosis in a dose and time dependent manner. The drugs mainly distributed in the cytoplasm. At 30 min after administration, the expressions of p-JNK and p-p38 in the treated group were significantly increased. Conclusion Lithospermum components can inhibit the proliferation of HepG 2 and B16F10 cells and induce apoptosis.