论文部分内容阅读
目的探讨系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血单核细胞核蛋白中可与序列特异DNA结合、具有基因调控能力的转录因子STAT1(signal transducer and activator of transcription 1,STAT1)的DNA结合活性和含量。方法对7例活动期SLE患者,6例病情稳定期SLE患者及8例健康人,采用凝胶滞留法(electrophoretic mobility shift assay,EMSA)检测外周血单核细胞核蛋白中转录因子STAT1的DNA结合活性,并对其进行定量。结果所有SLE患者外周血单核细胞核蛋白中转录因子STAT1的DNA结合活性和含量明显增高(P<0．05)。活动期SLE患者组外周血单核细胞核蛋白中转录因子STAT1的DNA结合活性和含量明显高于稳定期SLE患者组和正常人对照组(P<0．01)。稳定期SLE患者组也显著高于正常人对照组(P<0．05)。经统计学检验,转录因子STAT1的DNA结合活性和含量与患者的抗核抗体滴度、血沉相关;与补体C3浓度负相关;不与SLE疾病活动评分相关。结论SLE患者组外周血单核细胞核蛋白中转录因子STAT1的DNA结合活性和含量显著增高,这一结果提示转录因子STAT1的DNA结合活性的异常增强与SLE的发病有关。
Objective To investigate the DNA binding of signal transducer and activator of transcription 1 (STAT1), which can bind to sequence-specific DNA in peripheral blood mononuclear cells of patients with systemic lupus erythematosus (SLE) Activity and content. Methods DNA binding activity of transcription factor STAT1 in peripheral blood mononuclear cell nuclear protein was detected by electrophoretic mobility shift assay (EMSA) in 7 active SLE patients, 6 stable SLE patients and 8 healthy controls. , And quantify it. Results The DNA binding activity and content of transcription factor STAT1 in peripheral blood mononuclear cells were significantly increased in all SLE patients (P <0.05). The DNA binding activity and content of STAT1 in peripheral blood mononuclear cells of active SLE patients were significantly higher than those in stable SLE patients and normal controls (P <0.01). The stable SLE patients were also significantly higher than the normal control group (P <0.05). Statistically, the DNA binding activity and content of transcription factor STAT1 were correlated with antinuclear antibody titers and erythrocyte sedimentation rates, negatively correlated with complement C3 concentration, but not with SLE disease activity score. Conclusions The DNA binding activity and the content of STAT1 in peripheral blood mononuclear cell nucleoprotein of SLE patients are significantly increased. This result suggests that the abnormal increase of DNA binding activity of transcription factor STAT1 is related to the pathogenesis of SLE.