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标准的细胞遗传学技术须要分裂的细胞,分裂间的细胞不能进行分析,不能施行于分化末期的细胞(如分叶核中性白细胞),在低分裂率的细胞(如慢性淋巴性白血病)也难以应用,用染色体特异性探针原位杂交方法可以在分裂间期细胞和分化末期细胞中获得细胞遗传学信息.作者用9号染色体探针(158个碱基对,位于9qh区),以生物素化dUTP标志.标本的DNA用70%formamide变性,杂交后,用卵蛋白荧光素或碱性磷酸酶显色.每个标本镜下观察100-300个分裂间期和分化末期细胞.在已证实的5个单倍9号染色体的骨髓标本(4例ALL,1例慢粒母细胞危象)和5个三倍9号染色体(ALL,ANLL,MM各一例)的骨髓标本及(RAEB-t、真红/MDS各一例)的外周血标本中,其结果均符合.(作者以3个周围血、5个骨髓标本作纠正,以X+2SD算出,单倍染色体超过
Standard cytogenetic techniques require the division of cells, interstitial cells cannot be analyzed, cells that cannot be performed at the end of differentiation (such as leaf-dividing neutrophils), cells at low rates of division (such as chronic lymphocytic leukemia) It is difficult to apply, using chromosome-specific probe in situ hybridization method to obtain cytogenetic information in interphase cells and terminally differentiated cells. The authors used a chromosome 9 probe (158 base pairs located in the 9qh region) to Biotinylated dUTP markers. DNA of the specimens was denatured with 70% formamide, and after hybridization, visualized with albumin fluorescein or alkaline phosphatase. 100-300 interphase and late differentiation cells were observed under each microscope. Five bone marrow specimens (four ALL, one case of chronic myeloid crisis) and five triple chromosome 9 (ALL, ANLL, MM each) cases that have been confirmed on bone marrow specimens of RA 9 (RAEB In the peripheral blood samples of -t and true red/MDS, the results were consistent. (The author took 3 peripheral blood and 5 bone marrow specimens for correction, calculated with X+2SD, and the single chromosome exceeded