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目的了解小鼠胎肝干细胞是否具有向神经组织细胞分化的潜能。方法 采用免疫磁珠法分离雄性胎鼠肝脏的千细胞抗原1阳性(Sca-1+)细胞;将8-10周的C57BL/6J雌性小鼠用致死剂量(10.0Gy)放射线照射,然后尾静脉注射Sca-1+细胞2.0×103。Sca-1+细胞移植后2、4、6个月后,将受者脑组织作免疫组织化学和FISH双染色检测。结果移植后雌性受鼠脑组织内存在大量Y染色体阳性细胞;统计比较移植后2、4、6个月时,受者脑组织中Y染色体阳性细胞百分数无明显差别。免疫组织化学分析显示,这些Y染色体阳性细胞表达神经组织细胞特异标志,如神经元核特异蛋白(Neu N)、神经纤维细丝蛋白(NF-M)、微管蛋白Ⅲ(TuJ-1)或者胶质纤维酸性蛋白(GFAP) 等。Y染色体阳性细胞约占脑组织总细胞数的(4.5±0.5)%.其中Y染色体和Neu N均阳性的细胞为(1.2±0.3)%、Y染色体和GFAP均阳性的细胞为(1.0±0.2)%。结论经尾静脉移植小鼠胎肝Sca 1+细胞能迁移进入经致死剂量全身照射的雌性小鼠脑组织,并且能分化成神经组织细胞,且能长期存活(存活至少6个月以上)。这为神经组织细胞发育分化机制研究和神经系统疾病的治疗提供了理论依据和研究材料
Objective To understand whether mouse fetal liver stem cells have the potential to differentiate into nerve tissue cells. Methods C57BL / 6J female mice of 8-10 weeks were irradiated with lethal dose (10.0Gy) of radiotherapy and the caudal vein Sca-1 + cells injected 2.0 × 103. After 2, 4, and 6 months after Sca-1 + cell transplantation, the recipient’s brain tissue was examined by immunohistochemistry and FISH double staining. Results There were a large number of Y chromosome positive cells in the brain tissue of female rats after transplantation. There was no significant difference in the percentage of Y chromosome positive cells in recipients’ brain tissue at 2, 4 and 6 months after transplantation. Immunohistochemical analysis showed that these Y chromosome-positive cells expressed neuronal tissue-specific markers such as Neu N, NF-M, TuJ-1 or Glial fibrillary acidic protein (GFAP) and so on. The number of Y chromosome positive cells accounted for (4.5 ± 0.5)% of the total number of brain cells, with (1.2 ± 0.3)% positive cells on Y chromosome and Neu N, and 1.0 ± 0.2 on Y chromosome and GFAP )%. Conclusion Transplantation of mouse fetal liver Sca 1+ cells via the tail vein can migrate into the brain of mice exposed to lethal doses of whole body radiation and can differentiate into neural tissue cells with long-term survival (survival of at least 6 months). This provides a theoretical basis and research materials for the study of neural differentiation and differentiation of cells and the treatment of nervous system diseases