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应用超薄切片、负染色和蔗糖梯度超离心技术,在自然发病和人工感染的对虾肝、肠、淋巴样器官、鳃和肌肉组织中,发现并纯化了一种新杆状病毒.该病毒直径96～112nm,是已知对虾病毒中最粗的一种.中央是高电子密度的核心,外裹衣壳和囊膜,两膜之间有宽阔的间隙,这是已报道的任何一种对虾杆状病毒所没有的.纯化的病毒核衣壳表面有螺旋排列的亚单位,这也是本病毒特有的.主要靶细胞是淋巴样细胞,未在肝、肠和鳃上皮细胞中观察到,这与对虾杆状病毒无一例外地侵染某一种上皮细胞迥然不同.病毒仅在靶细胞核内增殖,不形成封入体或包涵体,它是一种迄今未曾报道的新病毒,称之为“淋巴样细胞核型杆状病毒”.
A new baculovirus was found and purified in the liver, intestine, lymphoid organs, gill and muscle tissues of naturally occurring and artificially infected shrimp by ultrathin sectioning, negative staining and sucrose gradient ultracentrifugation, 96 ~ 112nm, is the coarsest one of the known shrimp virus.Central is the core of high electron density, the outer shell and capsule capsule, there is a wide gap between the two films, which is reported in any of a pair of shrimp This is also unique to the virus, which is not present in baculoviruses, and has a helix-arranged subunit on the surface of the purified virus nucleocapsid.The main target cells are lymphoid cells that are not observed in liver, gut and gill epithelial cells, Unlike shrimp baculoviruses that invade a particular type of epithelial cell, the virus proliferates only in the nucleus of the target cell and does not form an enclosure or inclusion body, a new virus that has not been reported so far, calling it a “ Lymphoid cell nuclear baculovirus ”.