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氨茶碱是茶碱与乙二胺的复合物,遇光易变色。本文报道高压液相层析测定乙二胺的方法,在0.15～13.5μg/ml范围内呈线性关系,5次测定值变异系数为1%。使用岛津高压液相层析仪LC-2F型,检出波长254nm,Nucleosil 10C_(18)柱,流动相乙醇-水(85:15),流速0.8ml/分,样品注入量2微升。精密秤定氨茶碱原料粉约250mg,置50ml量瓶,加水溶解后稀释至刻度,精密吸取1ml加20ml乙醇,摇匀后备用。另精密吸取注射剂1ml加乙醇稀释至100ml,摇匀备用。精密秤定片子粉末相当氨茶碱250mg,加水50ml剧烈振摇提取30分钟,过滤,精确吸取滤液1ml加乙醇稀释至20ml摇匀备用。以上样品液各取2ml和乙二胺标准液的乙醇溶液(45μg/ml)
Aminophylline is a complex of theophylline and ethylenediamine, when the light easily discolored. In this paper, the method for the determination of ethylenediamine by high pressure liquid chromatography has a linear relationship in the range of 0.15 ~ 13.5μg / ml. The coefficient of variation for 5 times of determination is 1%. Shimadzu high pressure liquid chromatography LC-2F was used. The detection wavelength was 254nm. Nucleosil 10C 18 column was used. The mobile phase was ethanol-water (85:15). The flow rate was 0.8ml / min. Precision scale set aminophylline raw meal about 250mg, set 50ml volumetric flask, dissolved in water diluted to the mark, precision drawing 1ml plus 20ml ethanol, shake spare. The other precision injection 1ml ethanol diluted to 100ml, shake spare. Precise scale tablet powder Aminophylline 250mg, 50ml of water shake vigorously extract 30 minutes, filtered, and the filtrate was accurately draw 1ml ethanol diluted to 20ml shake spare. The above sample solution each take 2ml and ethylenediamine standard solution of ethanol (45μg / ml)