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Cold acclimation is associated with many metabolic changes that lead to an increase of freezing tolerance.In order to investigate the biochemical process of cold acclimation in Ammopiptanthus mongolicus,seedlings were acclimated at 2℃ under 16-h photoperiod(150 μmol·m-2·s-1 photosynthetically active radiation) for 14d.Freezing tolerance in seedlings increased after 14d of cold-hardening.Contents of protein,proline and solute carbohydrate in cotyledon increased after cold acclimation.Patterns of isozymes of superoxide dismutase(SOD),peroxidase,catalase and polyphenol oxidase(PPO) were investigated.The activities of SOD,peroxidase and PPO in cold acclimated plants were increased during cold-hardening.We deduced that compatible solutes and antioxidant enzymes play important roles in development of freezing tolerance during cold acclimation in this evergreen woody plant.
Cold acclimation is associated with many metabolic changes that lead to an increase of freezing tolerance. In order to investigate the biochemical process of cold acclimation in Ammopiptanthus mongolicus, seedlings were acclimated at 2 ° C under 16-h photoperiod (150 μmol · m -2 · s-1 photosynthetically active radiation) for 14d. Cold tolerance in seedlings increased after 14d of cold-hardening. Tents of protein, proline and solute carbohydrate in cotyledon increased after cold acclimation. Pattern of isozymes of superoxide dismutase (SOD), peroxidase, catalase and polyphenol oxidase (PPO) were investigated. Activities of SOD, peroxidase and PPO in cold acclimated plants were increased during cold-hardening. We deduced that compatible solutes and of enzymes enzymes play important roles in development of freezing tolerance during cold acclimation in this evergreen woody plant.