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利用荧光基因组原位杂交 (GISH)、生化标记和DNA分子标记技术对普通小麦 (TriticumaestivumL .)K型细胞质雄性不育保持系T9112 89的染色体组成进行了鉴定与分析。GISH鉴定和黑麦特异散布重复序列的检测结果表明 ,T9112 89的外源遗传物质来源于黑麦 ,黑麦 1RS上的微卫星引物SCM9扩增结果和醇溶蛋白酸性聚丙烯酰胺凝胶电泳 (A_PAGE)分析、低分子量谷蛋白的SDS_PAGE分析均表明 ,T9112 89所含的黑麦遗传物质来源于 1RS ;A_PAGE和SDS_PAGE分析及小麦 1BS上的微卫星引物的扩增结果则表明 ,T9112 89缺少 1BS染色体臂或 1BS末端片段。GISH鉴定结果还表明 ,T9112 89中有罗泊逊易位和小片段易位两种类型的杂交信号 ,说明T9112 89是一个异质群体 ,但其罗泊逊易位又不同于生产上大面积应用的 1BL 1RS易位 ,它可能是一种新的复杂易位形式。虽然T9112 89的小片段易位未能打破优异农艺性状与劣质蛋白基因的连锁 ,但这种小片段易位的获得将有利于小麦和黑麦的遗传研究 ,这种种质材料在育种上的应用价值也应优于罗泊逊易位。
The chromosomal composition of the T-type cytoplasmic male sterile maintainer line T9112 89 of common wheat (Triticum aestivum L.) was identified and analyzed by fluorescence in situ hybridization (GISH), biochemical markers and DNA molecular markers. The results of GISH identification and rye-specific repeats showed that the exogenous genetic material of T9112 89 originated from the amplification results of microsatellite primers SCM9 on rye and rye 1RS and the results of gliadin acid polyacrylamide gel electrophoresis A_PAGE analysis showed that the rye genetic material contained in T9112 89 was derived from 1RS. The results of A_PAGE and SDS_PAGE analyzes and microsatellite primers on wheat 1BS indicated that T9112 89 lacked 1BS Chromosome arm or 1BS terminal fragment. The results of GISH also showed that T9112 89 has two types of hybridization signals: ropisenzyme translocation and small fragment translocation, indicating that T9112 89 is a heterogeneous population, but its ropinantranslocation is different from the production of large area Applying the 1BL 1RS translocation, it may be a new form of complex translocation. Although the small fragment translocation of T9112 89 failed to break the linkage between excellent agronomic traits and poor protein genes, the gain of such small fragment translocations will benefit the genetic research of wheat and rye, and the application of this germplasm in breeding The value should also be better than the Robertson translocation.