磷酸聚核糖基核糖醇（PRP）是b型流感杆菌（Hib）菌苗的有效成分。PRP作为一种非T细胞依赖性免疫原不能在2岁以下幼儿中诱导免疫记忆。将PRP共价结合于某一载体蛋白制成结合菌苗,使之成为T细胞依赖性抗原,则可对任何年龄组人群均有效。PRP的稳定性是保证结合菌苗效力的重要因素。因此,建立定量检测PRP的有效方法,监测Hib结合菌苗中PRP的含量甚为重要。 Phosphoryl ribosyl ribitol (PRP) is an active ingredient of the b-type bacilli of Hib. PRP as a non-T cell-dependent immunogen can not induce immune memory in children under 2 years of age. The PRP covalently linked to a carrier protein made of combined vaccine, making it a T-cell-dependent antigen, can be effective in any age group. The stability of PRP is an important factor to ensure the efficacy of the combined vaccine. Therefore, it is very important to establish an effective method for quantitatively detecting PRP and to monitor the content of PRP in the Hib-binding bacterin.