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目的:探讨丹参酮ⅡA(TanⅡA)对局灶性脑缺血再灌注损伤大鼠脑组织单核细胞趋化蛋白-1(MCP-1)和肿瘤坏死因子-α(TNF-α)含量的影响。方法:将大鼠随机分为假手术组、缺血再灌注组、TanⅡA低剂量治疗组和TanⅡA高剂量治疗组,线栓法建立局灶性脑缺血再灌注模型。TanⅡA高、低剂量治疗组于术前连续灌胃给予高、低剂量TanⅡA3天,每天1次。各组于脑缺血90min再灌注24h进行2,3,5-三苯基氯化四氮唑(TTC)染色观察脑梗死体积,采用酶联免疫吸附试验(ELISA)法检测检测脑组织MCP-1和TNF-α含量的变化。结果:(1)TanⅡA治疗组脑梗死体积较缺血再灌注组减少,高、低剂量组之间差异亦具有显著性(P<0.05)。(2)与假手术组比较,缺血再灌注组脑组织MCP-1和TNF-α含量明显升高;与缺血再灌注组比较,TanⅡA高、低剂量治疗组脑组织MCP-1和NTNF-α含量均明显降低,高、低剂量组之间差异具有显著性(P<0.05)。结论:降低缺血再灌注损伤脑组织MCP-1和TNF-α含量,抑制再灌注损伤炎症反应,可能是TanⅡA发挥脑保护重要途径之一。
Objective: To investigate the effect of Tan IIA on the content of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α) in rats with focal cerebral ischemia-reperfusion injury. Methods: Rats were randomly divided into sham operation group, ischemia reperfusion group, TanIIA low-dose treatment group and TanIIA high-dose treatment group. The model of focal cerebral ischemia reperfusion was established by suture embolization. TanIIA high-dose and low-dose treatment groups were given intragastric administration of high and low doses of TanIIA for 3 consecutive days before surgery. The cerebral infarct volume was observed by 2,3,5-triphenyltetrazolium chloride (TTC) staining in each group at 90 min after cerebral ischemia and 24 h after reperfusion, and the brain tissue was detected by enzyme-linked immunosorbent assay (ELISA). 1 and changes in TNF-α content. Results: (1) The volume of cerebral infarction in TanIIA treatment group was lower than that in ischemia-reperfusion group, and the difference between high and low dose groups was also significant (P<0.05). (2) Compared with the sham group, the content of MCP-1 and TNF-α in the brain tissue of the ischemia-reperfusion group was significantly increased. Compared with the ischemia-reperfusion group, the MCP-1 and NTNF in the brain tissue of the high and low dose Tan IIA group -α content was significantly reduced, and the difference between high and low dose groups was significant (P <0.05). Conclusion: Decreasing the content of MCP-1 and TNF-α in brain tissues of ischemia-reperfusion injury and inhibiting the inflammatory reaction of reperfusion injury may be one of the important ways for TanIIA to exert brain protection.