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采用PCR扩增，聚丙烯酰胺凝胶电泳分析技术，调查中国成都汉族群体DIS1656、D851179、D9S302、D185535及D195253等5个STR基因座的等位基因频率分布。D1S1656检出11个等位基因，35种基因型；DSS1179检出9个等位基因，32种基因型；D95302检出12个等位基因，50种基因型；D185535检出7个等位基因，20种基因型；D195253检出8个等位基因，28种基因型。5个STR基因座基因型频率分布符合Hardy-weinberg平衡（P＞0．05）。个人识别机率（DP）为0．92～0．98。分析了二代3口之家的遗传模式，证明5个STR基因座均符合孟德尔遗传规律。5个STR基因座PCR扩增采用同一条件，方法简单、快速、灵敏、重复性好，可用于法科学亲子鉴定和个人识别。
PCR amplification and polyacrylamide gel electrophoresis were used to investigate allele frequency distribution of five STR loci such as DIS1656, D851179, D9S302, D185535 and D195253 in Han population of Chengdu, China. D1S1656 detected 11 alleles, 35 genotypes; DSS1179 detected 9 alleles, 32 genotypes; D95302 detected 12 alleles, 50 genotypes; D185535 detected seven alleles , 20 genotypes; D195253 detected 8 alleles, 28 genotypes. The frequency distribution of five STR loci was in accordance with Hardy-weinberg equilibrium (P> 0.05). The probability of personal identification (DP) is 0.92 ~ 0.98. The genetic pattern of the second generation of three families was analyzed to prove that all five STR loci are in accordance with Mendel’s genetic rule. Five STR loci PCR amplification using the same conditions, the method is simple, fast, sensitive, reproducible, and can be used for scientific paternity testing and personal identification.