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建立了大鼠血中紫杉醇ＨＰＬＣ检测法。血样用乙醚提取后在Ｃ１８柱上以甲醇-水-乙腈（４∶３∶３）为流动相，检测波长为２２７ nm，炔诺酮为内标条件下检测。线性范围为0.1～120.0 μg/ml，最低检测限为2.5 μg，萃取回收率大于80.0％，日内及日间ＲＳＤ皆小于3.0％。用本法对大鼠iv紫杉醇脂质体及紫杉醇注射液（10 mg/kg）后的药代动力学进行了研究。结果表明两者血浓经时曲线均符合二室模型，t1/2β分别为5.40 h和2.47 h，其它各参数也发生了明显变化。
A HPLC method for the determination of paclitaxel in rat blood was established. The blood samples were extracted with ether and detected on a C18 column with methanol-water-acetonitrile (4: 3: 3) at a detection wavelength of 227 nm and norethindrone as an internal standard. The linear range was 0.1-120.0 μg / ml, the minimum detectable limit was 2.5 μg, and the extraction recovery was more than 80.0%. The RSDs were less than 3.0% in both days and days. Pharmacokinetics of rat paclitaxel liposomes and paclitaxel injection (10 mg / kg) were studied by this method. The results showed that the two curves of blood concentration were in line with the two-compartment model, t1 / 2β were 5.40 h and 2.47 h, and other parameters also changed significantly.