常用的盐酸吡哆醇的测定法有萤光分光法、高氯酸非水滴定法及用2,6-二氯醌亚胺的分光光度测定法但这些方法的缺点是颜色不稳定,并且在发色前需预先经几步处理。本文介绍一种用重氮化的苯肼-4-磺酸试剂的比色法用以测定片剂和注射剂中及含维生素B_1和B_(12)的复合制剂中的盐酸吡哆醇含量。将试剂加至样品溶液中, Commonly used methods for the determination of pyridoxine hydrochloride are spectrofluorometry, non-aqueous titration of perchloric acid, and spectrophotometry using 2,6-dichloroquinoneimine. However, the disadvantages of these methods are color instability, Before the color needs a few steps beforehand. This paper presents a colorimetric method using diazotized phenylhydrazine-4-sulfonic acid reagent to determine the amount of pyridoxine hydrochloride in tablets and injections and in combination with vitamin B_1 and B_ (12). The reagent is added to the sample solution,