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针对水产养殖中抗生素滥用引起微生物耐药基因污染的现象,本实验以杭州市某水产养殖区底泥样品为研究对象,测定了底泥样品中抗生素含量,并从其中分离出74株可培养细菌.采用PCR方法对分离菌株中磺胺类、四环素类、喹诺酮类、氯霉素类抗性基因(antibiotic resistance genes,ARGs)和整合子基因进行了检测.结果表明,除tet A(四环素类)耐药基因未被检出外,其余3种耐药基因及整合子基因均被检出,其中qnr S(喹诺酮类)耐药基因的检出率最高,为50.00%.另外,利用16S r DNA序列分析技术将分离菌株鉴定为12个属、19个种,包括环境中多种常见土著细菌及部分致病菌,其中气单胞菌(Aeromonas spp.)数量最多(29株),占分离菌株的39.19%.掌握水产养殖区中ARGs的污染现状,对控制其传播、保障食品安全和保护微生态环境具有重要指导意义.
In view of the phenomenon of microbial resistance gene contamination caused by antibiotic abuse in aquaculture, this study took the sediment samples of an aquaculture area in Hangzhou as the research object, determined the antibiotic content in sediment samples, and isolated 74 strains of culturable bacteria The sulfonamides, tetracyclines, quinolones, antibiotic resistance genes (ARGs) and integron genes in the isolates were detected by PCR.The results showed that except Tet (tetracyclines) The other three kinds of resistance genes and integron genes were detected, of which qnr S (quinolone) resistance gene had the highest detection rate of 50.00% .In addition, the 16S r DNA sequence Analytical techniques identified the isolates as 12 genera and 19 species, including a number of common indigenous and partial pathogenic bacteria in the environment, with the highest number of Aeromonas spp. (29 strains) 39.19% .Controlling the status of ARGs pollution in aquaculture areas is of great guiding significance for controlling its spread, ensuring food safety and protecting the micro-ecological environment.