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[目的 ]验证参与卡氏肺孢子虫致病过程中 ,宿主炎性反应及其对肺功能的影响。 [方法 ]:将小鼠CD4+ T细胞耗竭或将小鼠CD4+ 和CD8+ T细胞都耗竭后 ,经气管接种卡氏肺孢子虫。观察在CD8+ T细胞缺如或存在的情况下 ,小鼠呼吸机能的改变和肺炎的程度。 [结果 ]耗竭CD4+ 和CD8+ T细胞后 ,小鼠虽发生PCP ,但呼吸频率无明显加快 ,肺组织损伤程度也较轻。相反 ,仅耗竭CD4+ T细胞保留CD8+ T细胞的小鼠的呼吸频率明显加快 ,肺内炎性细胞反应和肺组织损伤程度均较重。支气管肺泡灌洗液 (BALF)中的CD8+ T细胞和嗜中性白细胞的数量明显增多。 [结论 ]PCP的致病过程中 ,宿主的炎性细胞反应对肺功能有直接损伤作用 ,其中CD8+ T细胞似起主要作用。
[Objective] To verify the host inflammatory response and its effect on pulmonary function in the pathogenesis of Pneumocystis carinii. [Method] After mice CD4 + T cells were depleted or both mouse CD4 + and CD8 + T cells were depleted, Pneumocystis carinii was inoculated through the trachea. Observe changes in respiratory function and pneumonia in mice in the absence or presence of CD8 + T cells. [Result] Although PCP was produced in mice after depletion of CD4 + and CD8 + T cells, respiratory rate did not increase significantly and lung injury was also mild. In contrast, mice that only depleted CD8 + T cells depleted CD4 + T cells had a significantly higher respiratory rate and a greater degree of inflammatory cell reactions and lung damage in the lungs. The number of CD8 + T cells and neutrophils in bronchoalveolar lavage fluid (BALF) was significantly increased. [Conclusion] The host cell inflammatory cell reaction has a direct effect on lung function during the pathogenesis of PCP. CD8 + T cells play a major role.