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经过近几年的实际应用,结合我们不断总结、归纳出的实践经验,提出了一些合理化建议,供使用此标准者借鉴,同时也发现了许多问题,和同行们共同商讨。1溶液配制技术要点1.1所用化学试剂应为分析纯或合格的化学纯,该方法属生化方法,需用化学试剂配制多种操作溶液,化学试剂的纯度和质量直接影响到所配溶液的浓度,进一步影响到实验结果。例如:如果丙烯酰胺和甲叉双丙烯酰胺的纯度不够,在水中难以
After several years of practical application, combined with our continuous summary and induction of practical experience, put forward some rationalization proposals for the use of this standard for reference, but also found many problems, and colleagues to discuss. 1 Solution Preparation Technical Points 1.1 Chemical reagents used should be of analytical grade or qualified chemical pure, this method is a biochemical method, the need for a variety of chemical reagents formulated operating solution, the purity and quality of chemical reagents directly affect the concentration of the solution, Further affect the experimental results. For example: If acrylamide and methylene bisacrylamide purity is not enough, difficult in water