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目的:研究参芪扶正粉针剂的提取工艺和大孔树脂分离纯化工艺,为参芪扶正粉针剂的工业化生产提供参考。方法:正交设计优化药材提取工艺,采用D101大孔吸附树脂对药材提取液进行吸附纯化,并以黄芪甲苷及黄芪、党参总皂苷为考察指标综合评价。结果:最大吸附浓度应小于3.7mg·mL-1。上样量为7倍量树脂床体积,用4倍量柱体积水洗脱杂质,以70%的乙醇为洗脱溶媒,8倍柱体积洗脱效果最好。结论:本提取纯化工艺有效提取分离和纯化黄芪和党参中总皂苷,为工业化生产提供了数据。
Objective: To study the extraction process of Shenqi Fuzheng powder injection and the separation and purification of macroporous resin, to provide a reference for the industrialization of Shenqi Fuzheng injection. Methods: The orthogonal design was used to optimize the extraction process of medicinal herbs. The drug was extracted and purified by D101 macroporous adsorption resin. Astragaloside IV, Astragalus membranaceus and Codonopsis pilosula were used as the comprehensive evaluation indexes. Results: The maximum adsorption concentration should be less than 3.7mg · mL-1. The amount of sample volume is 7 times the volume of resin bed, with 4 times the amount of column volume of water eluting impurities, 70% ethanol as the elution solvent, 8 times the best column elution volume. Conclusion: This extraction and purification process can effectively extract, separate and purify the total saponins from Radix Astragali and Codonopsis pilosula, providing data for industrial production.