通过Tn5诱变技术,分离了植物青枯菌生理小种1号、3号的胞外蛋白输出功能丧失突变体。由于Tn5在其基因组单一的eep位点的插入,突变体失去了向培养滤液分泌产生胞外酶和胞外蛋白质的能力。用碱性磷酸酯酶基因PhoA作为报导基因,研究这些胞外蛋白穿越突变体细胞内膜,结果表明,这些胞外蛋白质可以穿过其内膜,但失去了穿越其外膜的能力。胞外多糖在植物体内和体外的产生没有受到eep基因位点突变的影响。该突变体失去了对番茄植株的致萎能力。 The loss-of-function mutants of extracellular protein of Ralstonia solanacearum physiological race 1 and 3 were isolated by Tn5 mutagenesis. Due to the insertion of Tn5 at its genomic single eep site, mutants lost the ability to secrete extracellular and extracellular proteins secreted into the culture filtrate. Using alkaline phosphatase gene PhoA as a reporter gene, the extracellular proteins were studied to cross the internode of the mutant cells. The results showed that these extracellular proteins could cross their intima but lose their ability to cross their outer membrane. The production of extracellular polysaccharides in plants and in vitro is not affected by mutations in the eep gene locus. This mutant loses its ability to induce wilt in tomato plants.