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为建立子宫颈癌的体外培养细胞系，以人子宫颈高分化鳞癌裸鼠移植瘤为材料进行体外培养，原代培养生长后行传代培养，并按照细胞系建立标准检测细胞的一系列生物学特性，并行克隆、无血清培养及对铂类抗癌药物的敏感性试验。结果：细胞维持培养２０个月，传代１５１代，生长稳定，群体倍增时间为３７．４６ｈ，细胞呈上皮镶嵌状贴壁生长，趋向复层生长，无接触抑制。超微结构显示具有典型的桥粒结构和较多的张力原纤维。染色体数目２～１２６，假２倍体占５０％，主流范围７１～１００条（５９％），结构为人类染色体。细胞的肿瘤标记物（ＥＲ、ＰＲ、ＣＥＡ、ＰｃＮＡ）及癌基因蛋白产物（ｐ５３、ｎｍ２３、ＣｅｒｂＢ－２）免疫细胞化学检测呈高表达，ＰＣＲ检测ＨＰＶ１６、１８均阴性，ＦＣＭ检测ＤＮＡ指数为１．５５。裸鼠移植瘤组织病理形态学与患者原始肿瘤一致，且ＨＰＶ１６阳性。细胞系已克隆出３个亚株，无血清培养成功，体外、体内对铂类抗癌药物均敏感。表明该细胞系符合建系标准，是一株新的子宫颈癌细胞系，为子宫颈癌的实验研究提供了理想的材料
To establish an in vitro cultured cell line for cervical cancer, a human cervical well-transplanted tumor xenograft in nude mice was used as a material for in vitro culture, a primary culture was grown, a subculture was performed, and a series of organisms were tested according to the cell line establishment criteria. Characteristics, parallel cloning, serum-free culture, and susceptibility testing of platinum anticancer drugs. RESULTS: The cells were maintained in culture for 20 months, passaged for 151 generations, and the growth was stable. The population doubling time was 37.46 hours. The cells showed epithelial mosaic growth, tending to multi-layer growth without contact inhibition. The ultrastructure shows a typical desmosome structure and more tensile fibrils. The number of chromosomes is 2-126, the number of pseudo-diploids is 50%, the main range is 71-100 (59%), and the structure is human chromosome. Immunocytochemical detection of tumor markers (ER, PR, CEA, PcNA) and oncogene protein products (p53, nm23, CerbB-2) was highly expressed in the cells. HPV16 and 18 were negative by PCR and the DNA index was 1 by FCM. .55. The histological morphology of transplanted tumor in nude mice was consistent with that of the patient’s original tumor and was positive for HPV16. Three sub-strains have been cloned from the cell line, and serum-free culture has been successful. They are sensitive to platinum anticancer drugs both in vitro and in vivo. It shows that this cell line meets the established standards and is a new cervical cancer cell line, providing ideal materials for the experimental study of cervical cancer.