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目的对19种藏药提取物进行体外抗呼吸道合胞病毒(respiratory syncytial virus,RSV)活性的筛选。方法采用细胞病变效应法(cytopathogenic effect,CPE)观察药物对Hep-2细胞的最大无毒浓度(TC0)和药物抑制RSV的致病变作用。结果从19种藏药中筛选出柳兰叶凤毛菊Saussurea epilobioidesMaxim提取物具有显著的抗RSV活性,其TC0大于500μg/ml,且在预防给药,直接灭活,给药治疗3种加药方式下均具有抗RSV活性,其中预防给药对RSV具有更好的抑制作用。结论通过对19种藏药提取物的筛选,发现柳兰叶凤毛菊提取物具有抗RSV的活性。
Objective To screen 19 kinds of Tibetan medicinal extracts against respiratory syncytial virus (RSV) in vitro. Methods The cytopathogenic effect (CPE) was used to observe the maximum non-toxic concentration (TC0) of Hep-2 cells and the pathogenicity of RSV. Results The extract of Saussurea epilobioidesMaxim was screened from 19 kinds of Tibetan medicines with significant anti-RSV activity. TC0 was more than 500μg / ml, and under the condition of prophylactic administration, direct inactivation and drug administration All have anti-RSV activity, of which the prophylactic administration has a better inhibitory effect on RSV. Conclusion Through the screening of 19 kinds of Tibetan medicine extracts, we found that the extract of Liriodendron amurense has anti-RSV activity.