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目的：建立高效液相色谱法测定环孢素Ａ的方法。方法：以环孢素Ｄ为内标。甲醇水（８０２０，Ｖ／Ｖ）为流动相，检测波长２１０ｎｍ。结果：本法ＣｓＡ与ＣｓＤ分离效果好。在５０～１０００μｇ／Ｌ范围线性良好，平均方法回收率为１０１．７８％，日内及日间精密度＜８％（ｎ＝５）。结论：本法简便、准确，能较好的满足临床血药浓度监测的需要。
Objective: To establish a method for the determination of cyclosporin A by high performance liquid chromatography. Methods: Cyclosporine D was used as internal standard. Methanol water (8020, V / V) as the mobile phase, detection wavelength 210nm. Results: This method CsA and CsD separation effect. The linearity was good in the range of 50-1000 μg / L with the average recovery of 101.78%. The intra- and inter-day precision was <8% (n = 5). Conclusion: This method is simple, accurate and can better meet the needs of clinical blood drug concentration monitoring.