利用原位ＤＮＡ末端转移酶标记技术和ＳＰ免疫组化方法检测肝硬变和肝细胞癌（ＨＣＣ）组织中凋亡细胞和增殖细胞核抗原。结果显示：ＨＣＣ中凋亡细胞密度显著低于肝硬变，而增殖细胞密度明显高于肝硬变。肝硬变中凋亡细胞主要分布于假小叶周边形成“凋亡细胞带”；在癌组织中呈散在分布。提示：肝硬变“凋亡细胞带”的形成可能与肝血流改变有关；肝硬变癌变过程中可能存在细胞选择性增殖 Apoptotic and proliferating cell nuclear antigens were detected in cirrhotic and hepatocellular carcinoma (HCC) tissues using in situ DNA terminal transferase labeling and SP immunohistochemistry. The results showed that the density of apoptotic cells in HCC was significantly lower than that of cirrhosis, and the proliferation cell density was significantly higher than that of cirrhosis. The apoptotic cells in cirrhosis mainly distributed in the periphery of pseudolobules to form “apoptotic cell bands,” and scattered in cancer tissues. Tip: The formation of “apoptotic cell band” in cirrhosis may be related to hepatic blood flow changes; there may be selective cell proliferation in the process of cirrhosis