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目的:建立高效液相色谱法测定调脂胶囊中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量。方法:采用Venusil C18色谱柱(4.6 mm×250 mm,5μm),以乙腈(A)-水(B)为流动相,四阶梯式梯度洗脱[0～35 min,A-B(19∶81);35～80 min,AB(29∶71);80～105 min,A-B(40∶60);105～120 min,A-B(19∶81)],流速1.0 mL·min-1,检测波长203 nm,柱温为室温。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1分别在2.21～13.28 mg、0.64～3.87 mg、2.80～16.82 mg范围内有良好的线性关系;平均回收率(n=9)分别为98.9%、97.4%、99.5%。结论:本方法结果准确,灵敏度高,重复性好,可作为调脂胶囊质量控制的方法。
Objective: To establish a method for the determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Tiaozhi Capsule by HPLC. Methods: Venusil C18 column (4.6 mm × 250 mm, 5 μm) was used with gradient elution of acetonitrile (A) - water (B) The detection wavelength was set to 203 nm at a flow rate of 1.0 mL · min-1 at 35 to 80 min, AB (29:71), 80 to 105 min, AB (40:60), 105 to 120 min, AB (19:81) The column temperature is room temperature. Results: The concentrations of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in the range of 2.21 ~ 13.28 mg, 0.64 ~ 3.87 mg and 2.80 ~ 16.82 mg, respectively. The average recoveries (n = 9) were 98.9% , 97.4%, 99.5%. Conclusion: The method is accurate, sensitive and reproducible. It can be used as a quality control method for Tiaozhi capsule.