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生长素应答因子(Auxin responsive factors)是植物内传导生长素信号、调控生长素响应的功能基因的表达过程中,起中心作用的重要转录因子,参与植物生长发育的各个方面的调控,包括组织分化、器官发生、顶端优势和根生成、趋向性等.我们利用豆科植物的模式作物,蒺藜苜蓿已有的全基因组序列数据,应用生物信息学的手段,对蒺藜苜蓿全基因组和天蓝苜蓿叶片转录组A RFs基因家族进行系统的分析和基因功能预测.共鉴定出34个完整的蒺藜苜蓿ARFs基因,其中27个基因有基因芯片的表达证据.天蓝苜蓿鉴定出1 1个完整的A RFs基因.染色体定位分析发现在蒺藜苜蓿第5染色体上聚集了6个基因结构和氨基酸序列均非常相似的A RFs.系统进化分析表明这6个基因在拟南芥中没有同源基因.拟南芥的部分ARFs在苜蓿中也没有同源基因.应用基因芯片数据进行基因表达分析表明蒺藜苜蓿的ARFs在不同器官特异性的表达.部分蒺藜苜蓿ARFs成员响应盐胁迫和菌根真菌侵染.Med仃8g100050不仅响应盐胁迫上调表达,菌根真菌侵染后也上调表达.“,”Auxin Responsive Factors (Auxin responsive factors) are important transcription factors that play a central role in the process of auxin signaling,auxin-responsive functional genes regulation in plants,and participate in the regulation of various aspects of plant growth and development,including tissue differentiation,organogenesis,apical dominance,root generation,tropism and so on.In this study,taking advantage of the whole genome sequence data ofMedicago truncatula,the ARFs gene family ofMedicago truncatula was systematically analyzed and the function of the genes was predicted by bioinformatics.A total of 34 complete ARFs genes from Medicago truncatula were identified,of which 27 genes had gene expression data.11 complete ARFs genes from Medicago lupulina L.,were identified.Chromosomal location analysis revealed that six ARFs with similar gene structure and amino acid sequence were clustered on chromosome 5 ofM.truncatula.Phylogenetic analysis showed that the six genes did not have homologous genes in Arabidopsis.Partial ARFs of Arabidopsis thaliana also had no homologous genes in M.truncatula.Gene expression analysis using gene chip data showed that ARFs of M.truncatula were expressed specifically in different organs.Some members of ARFs from Medicago truncatula responded to salt stress and mycorrhizal fungi infection.Medtr8g100050 not only responded to salt stress,but also was up-regulated after mycorrhizal fungi infection.