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为探讨UV-B辐射胁迫对大豆幼苗的伤害机制,采用水培法和模拟UV-B辐射方法研究紫外辐射(UV-B,280～320 nm)胁迫对大豆“台湾292”幼苗叶片含水量的影响。静态试验结果表明,UV-B辐射降低了大豆幼苗叶片的自由水含量,UV-B处理组自由水与束缚水比值(自由水/束缚水)低于CK组。动态试验结果表明,UV-B辐射期大豆幼苗叶片含水量下降,恢复期含水量逐渐提升。WV-B辐射对大豆幼苗叶片两种渗透调节物质(游离脯氨酸、可溶性糖)含量的影响不同,可使游离脯氨酸大量积累,而使可溶性糖含量急剧下降。游离脯氨酸及可溶性糖在低剂量UV-B辐射下对维持叶片含水量的作用明显;而在高剂量UV-B辐射下,细胞严重失水,脯氨酸和可溶性糖的渗透调节作用有限。研究表明,UV-B辐射会抑制大豆幼苗水分代谢。
In order to investigate the mechanism of injury to soybean seedlings under UV-B radiation stress, the effects of UV-B radiation (280-320 nm) on the leaf content of soybean in “Taiwan 292” seedling were studied by UV- The impact of water. The results of static experiments showed that UV-B radiation reduced the free water content of soybean seedling leaves, and the ratio of free water to irreducible water in UV-B treatment group was lower than that in CK group (free water / irreducible water). The results of dynamic experiments showed that the water content of leaves of soybean seedlings in UV-B radiation stage decreased and the water content in recovery stage increased gradually. The effects of WV-B radiation on the contents of two osmotic regulators (free proline and soluble sugar) of soybean seedling leaves were different, which could cause the accumulation of free proline and the drastic decrease of soluble sugar content. The effects of free proline and soluble sugar on maintaining leaf water content under low-dose UV-B radiation were obvious. Under high-dose UV-B radiation, the cells suffered from severe water loss and osmotic adjustment of proline and soluble sugar were limited . Studies have shown that UV-B radiation will inhibit soybean seedling water metabolism.