论文部分内容阅读
核型分析是诊断人类胚胎染色体异常的常规方法 ,但是通过细胞遗传学方法对人类种植前胚胎进行染色体检测并不容易。因此分裂间期细胞核的荧光原位杂交 (FISH)就成为首选方法之一。体外受精 (IVF)种植前胚胎的 FISH和核型分析均表明 :胚胎的染色体异常率可高达 2 5 %～ 5 1%。FISH分析显示染色体异常最常见的情况是染色体嵌合 (2 2 %～ 2 4 % )和胚胎分裂紊乱 (7%～ 2 6 % )。虽然目前还不能应用以上方法对自然受精的胚胎进行检测 ,但是染色体嵌合以及胚胎分裂紊乱较高的发生率可以为我们合理的解释人类的月经周期自然妊娠率为什么如此之低 (2 5 % ) ,提供理论依据
Karyotyping is a routine method of diagnosing chromosomal abnormalities in human embryos, but chromosomal testing of human preimplantation embryos by cytogenetics is not easy. Therefore, fluorescence in situ hybridization (FISH) of interphase nuclei becomes one of the preferred methods. FISH and karyotype analysis of preimplantation embryos in in vitro fertilization (IVF) showed that the rate of chromosomal aberrations in embryos could be up to 25% ~ 51%. FISH analysis revealed the most common chromosomal abnormalities were chromosome chimerism (22% -24%) and embryonic disruption (7% -26%). Although the above methods can not be used to detect naturally fertilized embryos, the higher incidence of chromosomal mosaicism and embryo disruption may provide a reasonable explanation for why the natural pregnancy rate in human menstrual cycles is so low (25%) , To provide a theoretical basis