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【目的】观察中药益气血补肝肾方对胚胎着床障碍小鼠子宫内膜整合素β3表达的影响,探讨其改善胚胎着床的分子基础。【方法】将90只雌鼠随机分为正常组、模型组和治疗组3组,每组30只,其中,治疗组小鼠予以中药益气血补肝肾方治疗(包括经后增殖方8 mg/kg和促黄体方8 mg/kg),在妊娠第4天(Pd4)上午8∶00采用米非司酮诱导模型组和治疗组小鼠胚胎着床障碍,12 h后处死小鼠(每组20只),剖腹取小鼠子宫,小心刮取子宫内膜,采用Real-time PCR和Western Blot的方法分别在m RNA和蛋白水平上检测整合素β3在各组小鼠子宫内膜中的表达情况。其余小鼠(每组10只)于Pd4剖腹取小鼠子宫,固定于体积分数2.5%戊二醛溶液中,行扫描电镜观察。【结果】Real-time RT-PCR和Western Blot结果显示:模型组整合素β3 m RNA与蛋白表达显著低于正常组(P<0.05);治疗组可显著升高整合素β3 m RNA与蛋白表达水平,与模型组比较,差异均有统计学意义(P<0.05)。胞饮突在模型组中的表达少于治疗组和正常组,而胞饮突在治疗组和正常组的表达相似。【结论】中药益气血补肝肾方可上调子宫内膜中整合素β3的表达,对胞饮突在Pd4小鼠子宫内膜中的表达有正调节作用。
【Objective】 To observe the effect of Yiqixue Bugan Decoction on endometrial integrin β3 expression in embryo implantation dysfunction mice and to explore its molecular basis for improving embryo implantation. 【Methods】 Ninety female mice were randomly divided into three groups: normal group, model group and treatment group, with 30 rats in each group. The mice in the treatment group were treated with Yiqi Xue Bugan Hegan Decoction mg / kg and luteinizing side 8 mg / kg). Mice were implanted with mifepristone at 8:00 am on the 4th day of gestation (PD4), and mice were sacrificed 12 h later Each group of 20), take the cesarean section of the uterus, carefully scrape the endometrium, using Real-time PCR and Western Blot method were detected in the m RNA and protein levels of integrin β3 in the mouse endometrium The expression of the situation. The remaining mice (10 in each group) were harvested from the uterus in a Pd4 cadaver and fixed in 2.5% glutaraldehyde solution for scanning electron microscopy. 【Results】 The results of Real-time RT-PCR and Western Blot showed that the expression of integrin β3 m RNA and protein in the model group was significantly lower than that in the normal group (P <0.05); the treatment group could significantly increase the integrin β3 m RNA and protein expression Level, compared with the model group, the difference was statistically significant (P <0.05). The expression of pinopodes in the model group was less than that in the treatment group and the normal group, while the expression of pinopodes in the treatment group and the normal group was similar. 【Conclusion】 Yiqi Xue Bugan Hegan can up-regulate the expression of integrin β3 in endometrium, and has a positive effect on the expression of pinopodes in the endometrium of Pd4 mice.