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目的研究转化生长因子β1(TGF-β1)基因多态性与高级别宫颈上皮内瘤变(CINⅡ-Ⅲ)遗传易感性的关系。方法选择191例CINⅡ-Ⅲ患者为病例组,207例年龄相匹配的正常者为对照组,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测TGF-β1基因C509T和T869C多态性在两组中的频率和分布,比较不同基因型携带者患CINⅡ-Ⅲ的危险性。结果与携带T869CTT基因型相比,携带T869CTC/CC基因型者罹患CINⅡ-Ⅲ的风险升高1.582倍(OR=1.582,95%CI=1.026-2.438,P<0.05)。产次0-1次和不吸烟的女性携带T869CTC/CC基因型较携带TT基因型者罹患CINⅡ-Ⅲ的风险升高更为显著(P<0.05)。结论 TGF-β1T869C多态性可能与人群CINⅡ-Ⅲ的遗传易感性相关。
Objective To investigate the relationship between the genetic polymorphism of transforming growth factor β1 (TGF-β1) gene and the susceptibility to high-grade cervical intraepithelial neoplasia (CINⅡ-Ⅲ). Methods A total of 191 patients with CINⅡ-Ⅲ were selected as the case group and 207 age-matched normal controls as the control group. The levels of C509T and TGF-β1 mRNA and protein were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) T869C polymorphism in the frequency and distribution of the two groups to compare the risk of different genotype carriers suffering from CIN Ⅱ-Ⅲ. Results Compared with the T869CTT genotype, the risk of CINⅡ-Ⅲ was 1.582 times higher in patients with T869CTC / CC genotype (OR = 1.582, 95% CI = 1.026-2.438, P <0.05). The T869CTC / CC genotypes in women who received 0-1 times of smoking and those who did not smoke were more likely to have CINⅡ-Ⅲ than those who had TT genotype (P <0.05). Conclusion The polymorphism of TGF-β1T869C may be related to the genetic predisposition of CINⅡ-Ⅲ.