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为弄清医源性感染的常见原因和类型,作者在1980年3月1日～1981年2月28日期间对Buffalo儿童医院作了前瞻性研究。研究方法专人负责查阅住院记录,提出病毒学或细菌学诊断试验申请单,收集细菌、霉菌、病毒培养及快速诊断的阳性报告,根据美国疾病控制中心(CDC)标准确定是否医源性感染及其分类。其中对病毒作如下修改,即首发症状出现于住院6天以后的呼吸道病毒感染,或出现于住院4～6天的消化道病毒感染,应考虑为医源性感染,因此时间超过了呼吸道合胞病毒(RSV)及轮状病毒的潜伏期。病毒感染至少被下列一种以上的方法证明:(1)用聚乙稀管吸取鼻咽部分泌物,接种于标准的内皮细胞,人胚肺成纤维细胞和原代猴肾培养细胞;(2)上述标本还用于免疫荧光抗体技术分析,查病毒抗原;(3)电镜检查大便中病毒颗粒。
To understand the common causes and types of iatrogenic infections, the authors conducted a prospective study of Buffalo Children’s Hospital between March 1, 1980 and February 28, 1981. The research staff were responsible for checking inpatient records, submitting an application form for virological or bacteriological diagnostic tests, collecting positive reports of bacteria, molds, virus culture and rapid diagnosis, and determining if the iatrogenic infection was based on the CDC criteria classification. Which made the following changes to the virus, the first symptom occurred in 6 days after admission of respiratory virus infection, or appeared in hospital 4 to 6 days of gastrointestinal virus infection should be considered as iatrogenic infection, so the time exceeds the respiratory syncytial Virus (RSV) and rotavirus latency. Viral infection is demonstrated by at least one of the following methods: (1) Nasopharyngeal secretions are aspirated into polyethylene tubes, seeded on standard endothelial cells, human embryonic lung fibroblasts, and primary monkey kidney cells; (2 ) The above specimens are also used for immunofluorescent antibody analysis of antibodies, check the virus antigen; (3) electron microscopy stool virus particles.