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背景:脑出血患者常遗留严重的神经功能缺损。目前关于其发病机制的研究日益深入,希望能找到改善预后的有效方法。目的:经脑内血肿腔局部应用神经节苷脂(Ganglioside)GM-1,探讨脑内局部给药能否有减低药量而提高疗效的效果,同时脑内局部用药是否具有局部量效关系。设计:随机对照的实验研究。单位:西安交通大学第二医院神经外科、设备科。对象:实验于2000-08/2001-04在西安交通大学医学院神经生物研究中心完成。选择健康雄性SD大鼠,体质量250～300g,由陕西中医研究院实验动物中心提供。干预:将大鼠随机分为4组:①假手术组(12只):行右侧尾状核穿刺。②模型组(12只):于右侧尾状核注入50μL未抗凝自体血。③局部给药组:分为大、中、小剂量组(每组12只)。制作模型后6h分别按照2mg/kg,0.8mg/kg和0.32mg/kg于脑内血肿腔注入GM-1,液量按2mg/kg计算,不足部分用对照液(即GM1注射液溶剂,按说明书配制)补齐,同时经尾静脉注入相等体积对照液。④全身给药组(12只):制作模型后6h经尾静脉注入GM-12mg/kg,同样体积对照液注入右侧尾状核。上述各组于脑出血后72h处死,测定脑含水量(每组取8只);行组织学检查(每组取4只),并作相对损失神经元计数。主要观察指标:①脑水含量。②组织学观察。③相对损失神经元计数。结果:脑内局部
Background: Patients with ICH often have serious neurological deficits. At present, the research on its pathogenesis is deepening, hoping to find an effective way to improve the prognosis. OBJECTIVE: To investigate whether topical administration of Ganglioside GM-1 can effectively reduce the dose of intracerebral hemorrhage and improve the curative effect of intracerebral hematoma. Design: Randomized controlled experimental study. Unit: Second Hospital of Xi’an Jiaotong University, Department of Neurosurgery, Equipment. PARTICIPANTS: Experiments were performed at the Neurobiology Research Center, Xi’an Jiao Tong University School of Medicine from August 2000 to April 2001. Select healthy male SD rats, body weight 250 ~ 300g, provided by the Experimental Animal Center of Shaanxi Academy of Traditional Chinese Medicine. Intervention: The rats were randomly divided into 4 groups: ① sham operation group (12): the right caudate nucleus puncture. ② model group (12): on the right caudate nucleus injection of 50μL non-anticoagulant blood. ③ local administration group: divided into large, medium and small dose group (12 in each group). Six hours after the model was established, GM-1 was injected into the intracerebral hematoma cavity at 2mg / kg, 0.8mg / kg and 0.32mg / kg, respectively. The amount of liquid was calculated as 2mg / kg. Manual preparation) fill, at the same time through the tail vein into the same volume of reference solution. ④ systemic administration group (12): 6h after the model was injected through the tail vein GM-12mg / kg, the same volume of control fluid into the right caudate nucleus. The above groups were sacrificed at 72 hours after intracerebral hemorrhage and the brain water content was measured (8 in each group). Histological examination (4 in each group) was performed and the relative loss of neurons was counted. MAIN OUTCOME MEASURES: ① brain water content. ② histological observation. ③ relative loss of neurons count. Results: Local brain