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目的:对抗-HCV ELISA灰区献血者进行追踪检测,了解其真实的病毒感染情况,探讨灰区范围设置的理论依据及合理的血站筛查模式。方法:对抗-HCV ELISA灰区献血者在献血间隔3个月、6个月时采集标本进行ELISA试验、HCV PCR检测及重组免疫印迹试验(RIBA)确证3种平衡检测。结果:本次追踪研究的34例灰区标本中,其中1例核酸检测值继续上升,ELISA和RIBA检测转为阳性,证实为当时献血者正处于病原体感染标志物的窗口期;另1例献血时核酸检测阳性,后经过追踪试验仍为阴性,证实为核酸实验时的污染。结论:灰区内的血液存在传染病毒的安全隐患,应合理设置灰区范围,对灰区内的血液进行报废处置及献血信息屏蔽处理是非常有必要的;建议各地按照国家要求尽快开展核酸检测,在献血者中开展ELISA和核酸蛋白平行检测,可以缩短感染窗口期,有效降低输血传播感染病毒的风险。
OBJECTIVE: To test the real-time virus infection of anti-HCV ELISA gray zone blood donors and to explore the theoretical basis of gray zone setting and rational blood-screening method. Methods: The anti-HCV ELISA gray blood donors were collected at intervals of 3 months and 6 months after blood donation for ELISA test, HCV PCR test and recombinant immunoblot test (RIBA) to confirm the three balance tests. RESULTS: Of the 34 gray-zone samples studied in this follow-up study, the detection value of nucleic acid in one case continued to increase, and the ELISA and RIBA tests turned positive, confirming that blood donors were at the window of pathogen infection markers at that time. Another case of blood donation When the nucleic acid test positive, after the follow-up test is still negative, confirmed that the nucleic acid experiment pollution. Conclusion: There is a potential safety hazard of infectious virus in the blood in the gray zone. It is necessary to dispose the gray zone in a reasonable way, to discard the blood in the gray zone and to shield the blood donation information. It is suggested that nucleic acid detection should be carried out as soon as possible according to the national requirements , In blood donors to carry out ELISA and nucleic acid protein parallel detection, can reduce the window of infection and effectively reduce the risk of transmission of transmitted virus infection.