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目的 探讨非小细胞肺癌患者外周血淋巴细胞中CD4 4和CD54 表达与临床病理的关系。方法 应用流式细胞术对 5 0例肺癌患者外周血淋巴细胞中CD4 4和CD54 表达进行荧光免疫检测 ,并与正常对照组 (30名 )及肺部良性病变组 (2 5例 )进行对比研究。结果 5 0例肺癌患者外周血淋巴细胞中CD4 4和CD54 表达明显高于正常对照组及良性病变组 (P <0 0 1)。良性病变组和正常对照组之间CD4 4和CD54 的表达比较 ,差异无显著性 (P >0 0 5 )。肺癌伴淋巴结转移CD4 4和CD54 高于不伴淋巴结转移者 (P <0 0 1) ;Ⅲ期、Ⅳ期和Ⅰ期、Ⅱ期之间CD4 4表达比较 ,差异有显著性 (P <0 0 1) ;Ⅳ期和Ⅰ期、Ⅱ期、Ⅲ期之间CD54 表达比较 ,差异有显著性 (P <0 0 1)。CD4 4和CD54 表达与肺癌组织学分级有明显相关性 (P <0 0 5或 0 0 1) ;与鳞癌和腺癌没有相关性。结论 应用流式细胞仪检测CD4 4和CD54 的表达水平可作为肺癌转移和预后的指标
Objective To investigate the relationship between the expression of CD4 4 and CD54 in peripheral blood lymphocytes and clinicopathology in patients with non-small cell lung cancer. Methods Fluorescent immunodetection of CD4 4 and CD54 expression in peripheral blood lymphocytes of 50 lung cancer patients was performed by flow cytometry, and compared with normal control group (30 patients) and lung benign disease group (25 patients). . Results The expression of CD4 4 and CD54 in peripheral blood lymphocytes of 50 patients with lung cancer was significantly higher than that of normal controls and benign lesions (P < 0 01). There was no significant difference in the expression of CD4 4 and CD54 between benign lesions and normal controls (P > 0.05). CD4 4 and CD54 in patients with lung cancer with lymph node metastasis were higher than those without lymph node metastasis (P <0 01). There was a significant difference in CD4 4 expression between stage III, IV and stage I and II (P <0. 1) There was a significant difference in CD54 expression between stage IV and stage I, stage II, and stage III (P < 0.01). The expression of CD4 4 and CD54 was significantly correlated with the histological grade of lung cancer (P <0 05 or 0 01); there was no correlation between CD4 4 and CD54 expression and squamous cell carcinoma and adenocarcinoma. Conclusion Using flow cytometry to detect the expression of CD4 4 and CD54 can be used as an indicator of metastasis and prognosis of lung cancer