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[目的]研究胃癌组织中紧密连接蛋白1(ZO-1)的表达及意义。[方法]采用免疫组织化学方法检测辽宁省肿瘤医院2001年1月~2006年12月期间收集的50例正常胃组织和100例胃癌及其癌旁组织标本中ZO-1表达情况,并分析胃癌组织中ZO-1表达水平与各临床参数之间的关系。[结果]ZO-1在胃癌组织中表达下调,在胃癌组织中的表达率(40.0%)显著低于癌旁组织(84.0%)和正常胃组织(84.0%)(P=0.000)。ZO-1表达水平与胃癌患者年龄、性别和组织学类型无关(P均>0.05)。ZO-1表达水平与分化程度、浸润深度、区域淋巴结转移、远处转移及临床分期具有相关性,分化程度越低、浸润越深、伴有淋巴结转移和远处转移、临床分期越高,ZO-1表达水平越低(P均<0.05)。ZO-1高表达组5年生存率高于低表达组(70%vs20%,P<0.05)。[结论]ZO-1在胃癌组织中表达下调,与肿瘤的临床分期、转移和预后密切相关,可作为胃癌诊断和预后评价的标志物之一。
[Objective] To study the expression and significance of tight junction protein 1 (ZO-1) in gastric cancer. [Methods] Immunohistochemistry was used to detect the expression of ZO-1 in 50 specimens of normal stomach tissue and 100 specimens of gastric carcinoma and paracancerous tissues collected from Liaoning Provincial Tumor Hospital from January 2001 to December 2006. The relationship between ZO-1 expression levels and clinical parameters in the tissue. [Results] The expression of ZO-1 was down-regulated in gastric cancer tissues. The expression rate of ZO-1 in gastric cancer tissues (40.0%) was significantly lower than that in para-cancer tissues (84.0%) and normal gastric tissues (84.0%) (P=0.000). The expression level of ZO-1 was not related to the age, sex and histological type of gastric cancer patients (P>0.05). The expression level of ZO-1 was related to the degree of differentiation, infiltration depth, regional lymph node metastasis, distant metastasis and clinical stage. The lower the degree of differentiation, the deeper the invasion, the accompanied with lymph node metastasis and distant metastasis, the higher the clinical stage, ZO The lower the expression level of -1 (P <0.05). The 5-year survival rate of the ZO-1 high expression group was higher than that of the low expression group (70% vs 20%, P<0.05). [Conclusion] The down-regulation of ZO-1 in gastric cancer tissues is closely related to the clinical stage, metastasis and prognosis of tumors. It may be one of the markers for the diagnosis and prognosis evaluation of gastric cancer.