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为了明确肠道双歧杆菌数量是否会影响儿童对脊髓灰质炎病毒的免疫反应,笔者进行了一项随机的安慰剂对照试验。从出生到4月龄,给予婴儿服用发酵剂儿童配方(FIF)或标准配方(安慰剂)。每月测量儿童粪便中的双歧杆菌的数目。在接种前及第2次疫苗注射后1 个月,对抗脊髓灰质炎病毒IgA抗体对Pentacoq的反应进行评价。共随机抽取30名儿童,有20名完成了研究(安慰剂组9名,FIF组11名)。FIF组中粪便双歧杆菌水平在4月龄时显著升高(P=0.0498)。而且FIF组婴儿在第4月龄时长双歧杆菌/婴儿双歧杆菌携带量较高(P=0.0399)。经过Pentacoq激发(P<0.001) 后,FIF组的抗脊髓灰质炎病毒IgA滴度显著升高(P< 0.02)。抗体滴度与双歧杆菌数目尤其与长双歧杆菌/ 婴儿双歧杆菌和短双歧杆菌数目(P<0.02)密切相关。体内寄生长双歧杆菌/婴儿双歧杆菌的婴儿同样显示具有较高的抗脊髓灰质炎病毒IgA水平(P< 0.02)。综上所述,目前的结果显示婴儿的抗脊髓灰质炎病毒反应能通过服用发酵配方所激发,该配方有利于肠道双歧杆菌生长。这种对免疫系统的作用是来源于配方中双歧杆菌(主要是长双歧杆菌/婴儿双歧杆菌和短双歧杆菌的激发)的作用还是牛奶发酵所产生的复合物(例如缩氨酸)的作用尚待研究。
To determine whether the amount of bifidobacteria in the gut affects the immune response in children to poliovirus, I conducted a randomized placebo-controlled trial. From birth to 4 months of age, infants are given starter cultures for children (FIF) or standard formula (placebo). The number of Bifidobacteria in children’s stools is measured monthly. The response of poliovirus IgA antibodies to Pentacoq was evaluated before vaccination and one month after the second vaccination. Thirty children were randomly selected and 20 completed the study (9 in the placebo group and 11 in the FIF group). Bifidobacterium fecalis levels in the FIF group were significantly elevated at 4 months of age (P = 0.0498). Furthermore, Bifidobacterium / Bifidobacterium infantis was carried more frequently at 4 months of age in infants of FIF group (P = 0.0399). After Pentacoq challenge (P <0.001), anti-polio IgA titers in FIF group were significantly increased (P <0.02). The antibody titer and the number of Bifidobacteria are especially closely related to the numbers of Bifidobacterium longum / Bifidobacterium infantis and Bifidobacterium breve (P <0.02). Infants of B. parasitica Bifidobacterium / Bifidobacterium infantis also showed higher levels of anti-polio IgA (P <0.02). Taken together, the current results show that the infant’s anti-poliovirus response can be stimulated by taking a fermentation formulation that favors the growth of intestinal Bifidobacteria. This effect on the immune system is derived from the action of Bifidobacteria (mainly Bifidobacterium longum / Bifidobacterium infantis and Bifidobacterium breve) in the formulation or the complex produced by milk fermentation (eg, peptides ) Role remains to be studied.