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目的 观察抗癌药物长春新碱对体外培养成骨细胞的作用。方法 在新生SD大鼠头颅骨次代成骨细胞 (OB2 )培养液中分别加入不同浓度 (10 -1～ 10 -9g/L)长春新碱 ,分别观察OB2 的增殖功能 (用波长 5 70nm处OD值表示 )、分化功能 [用碱性磷酸酶 (ALP)活性表示 ]和矿化功能 (用矿化结节数 /视野表示 )。结果 OD值实验组为 0 0 91± 0 0 0 5～ 0 2 97± 0 0 44 ,对照组为 0 34 7± 0 0 35 ;ALP(U/g蛋白质 )活性实验组为 77± 12 ,对照组为 81± 4;矿化结节数 /视野 (个 )实验组为 1 0±0 816,对照组为 1 5± 1 0。结论 从绝对数来看 ,各浓度长春新碱对OB2 的增殖、分化和矿化功能均具抑制作用。与对照组比较 ,长春新碱对OB2 增殖功能的抑制作用具有极显著性意义 (P <0 0 1)。
Objective To observe the effect of anticancer drug vincristine on cultured osteoblasts in vitro. Methods Vincristine with different concentration (10 -1 ~ 10 -9 g / L) was added to the culture medium of newborn SD rats primary skull osteoblast (OB2) respectively to observe the proliferation function of OB2 (OD570nm OD Value), differentiation function [expressed as alkaline phosphatase (ALP) activity] and mineralization function (expressed as number of mineralized nodules / field of view). Results The OD value of the experimental group was 0 910050970044, the control group was 0 347 0 035; the activity of ALP (U / g protein) was 77 12 in the experimental group Group was 81 ± 4; mineralized nodules / field of view (a) experimental group was 10 ± 0 816, the control group was 15 ± 10. Conclusions In absolute terms, vinblastine at various concentrations has an inhibitory effect on the proliferation, differentiation and mineralization of OB2. Compared with the control group, vincristine has a very significant inhibitory effect on the proliferation of OB2 (P <0.01).