用兔抗人因子Ⅷ相关抗原(ⅧR:Ag)的抗血清检测正常人、山羊、兔、大鼠和纯系小鼠(C_(57)BL、LACA、AMM)血浆ⅧR:Ag,用间接免疫荧光抗体作人和各动物组织(肝、肾、小肠、脾)的ⅧR:Ag免疫组织化学定位。结果在人和兔血浆中出现明显电泳峰,肝、肾、小肠,脾组织血管内皮呈强荧光反应。在山羊,大鼠和小鼠各种系的血浆和组织的血管内皮中未测出阳性反应。 Normal human goats, rabbits, rats and pure mice (C_ (57) BL, LACA, AMM) were used to detect the plasma Ⅷ R: Ag with the antiserum of rabbit anti - human factor Ⅷ related antigen Fluorescent antibodies were used as ⅧR: Ag immunohistochemical localization in human and animal tissues (liver, kidney, small intestine, spleen). The results in human and rabbit plasma electrophoresis showed significant peaks, liver, kidney, small intestine, spleen tissue vascular endothelial showed a strong fluorescence reaction. No positive reaction was detected in the vascular endothelium of plasma and tissues in various lines of goat, rat and mouse.