选用Bouin固定液固定新鲜脊髓,石蜡包埋切片,采用普通的苏木素一伊红染色,清晰地显示了神经细胞胞浆内的嗜碱性物质一尼氏体,而且较特染简单、经济、不易退色.1材料与方法取狗新鲜脊髓颈膨大固定于Bouin液24小时.(1)苦味酸75份(饱和),甲醛25份,冰醋酸5份(用前临时配制);(2)常规脱水包埋;(3)石蜡切片5μm; (4)HE染色;(5)中性树胶封片.2结果尼氏体呈兰色粒状和块状,清晰可见,背景呈浅红色(附图). Bouin fixative was used to fix the fresh spinal cord and embedded in paraffin. The sections were stained with common hematoxylin and eosin, which clearly showed a basophilic-nitrided substance in the cytoplasm of nerve cells. Fade .1 Materials and Methods Take fresh dog spinal dilation fixed in Bouin solution for 24 hours. (1) picric acid 75 (saturated), 25 parts of formaldehyde, 5 parts of glacial acetic acid (pre-use preparation); (2) conventional dehydration Embedded; (3) paraffin section 5μm; (4) HE staining; (5) Neutral gum seal .2 Results Nissl body was blue granular and massive, clearly visible, the background was light red (with photos).