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[Objective]To optimize the extraction process of polyphenols from Punica granatum Linn.peel and discuss the antibacterial activity of the crude extracts.[Method]Microwave-assisted extraction(MAE) was used to extract polyphenols,and the extraction process was optimized by employing both the single-factor test and orthogonal test.The inhibitory activity of the crude extracts on Staphylococcus aureus,Proteus vulgaris and Bacillus cereus were compared.[Result]The optimum extraction conditions were as follows:screen of 60-80 mesh,extraction power of 385 W,solid-liquid ratio of 1:25(W/V,the same as following),extraction duration of 120 s and extraction times of 3,under which the yield of polyphenol from Punica granatum Linn.peel was 21.4%.The inhibitory activity of 0.96 mg/ml extracts on Staphylococcus aureus,Proteus vulgaris and Bacillus cereus were the strongest,the corresponding diameter of inhibition zone were 1.15,1.10 and 0.95 cm respectively and the corresponding MIC were 0.80,1.60 and 0.40 g/ml respectively.[Conclusion]The natural medicine was extracted from Punica granatum Linn.peel,which provided theoretical evidence for exploiting natural bacteriostat.
[Objective] To optimize the extraction process of polyphenols from Punica granatum Linn. Peel and discuss the antibacterial activity of the crude extracts. [Method] Microwave-assisted extraction (MAE) was used to extract polyphenols, and the extraction process was optimized by employing both the single-factor test and orthogonal test. The inhibitory activity of the crude extracts on Staphylococcus aureus, Proteus vulgaris and Bacillus cereus were compared. [Result] The optimum extraction conditions were as follows: screen of 60-80 mesh, extraction power of 385 W, solid-liquid ratio of 1:25 (W / V, the same as following), extraction duration of 120 s and extraction times of 3, under which the yield of polyphenol from Punica granatum Linn. inhibitory activity of 0.96 mg / ml extracts on Staphylococcus aureus, Proteus vulgaris and Bacillus cereus were the strongest, the corresponding diameter of inhibition zone were 1.15, 1.10 and 0.95 cm respectively and the corresponding MIC were 0.80, 1.60 an d 0.40 g / ml respectively. [Conclusion] The natural medicine was extracted from Punica granatum Linn. peel, which provided theoretical evidence for exploiting natural bacteriostat.