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目的:探讨腹部火器伤肠管穿透后核因子-κB在肺组织细胞凋亡中的作用,了解腹部火器伤肠管穿透后继发性肺损伤机制。方法:健康长白仔猪42头随机等分为对照组和伤后1h、2h、4h、8h、12h和24h组,实验组建立腹部火器伤肠管穿透模型后,用免疫组化图像分析法测定各组肺组织内NF-κB活性,同时用TUNEL测定肺组织细胞凋亡变化情况。结果:伤后各组肺组织内NF-κB活性明显高于对照组,并于伤后8h出现高峰。肺组织细胞凋亡指数伤后显著增高(P<0.05),并与NF-κB活性变化基本一致。结论:腹部火器伤肠管穿透后肺组织内NF-κB活性增强,肺细胞凋亡增多,从而继发导致肺损伤。
Objective: To investigate the role of nuclear factor-κB in the apoptosis of lung tissue after intestinal perforation of firearm in abdomen, and to understand the mechanism of secondary lung injury after intestine penetration in abdominal firearm wound. METHODS: Forty-two healthy Landrace piglets were randomly divided into control group and 1h, 2h, 4h, 8h, 12h and 24h after injury. The model of intestinal perforation after firearm was established in the experimental group, Group NF-κB activity in lung tissue, while TUNEL determination of lung tissue apoptosis changes. Results: The activity of NF-κB in the lung tissue of each group was significantly higher than that of the control group after injury, and peaked at 8h after injury. The apoptosis index of lung tissue was significantly increased after injury (P <0.05), and it was basically consistent with that of NF-κB. CONCLUSION: The NF-κB activity in the lung tissue increases after the intestine penetrates through the abdominal firearm wound and lung cell apoptosis increases, leading to secondary lung injury.