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目的探讨中药复方液对麻黄素致损伤的影响。方法采用递增剂量连续腹腔注射浓度为2.0g/L、3.0g/L和4.0g/L的麻黄素溶液,在注射麻黄素1h后再分别灌胃,浓度为20.0g/L、30.0g/L和40.0g/L的中药复方液。分别于灌胃中药5、10、15d后,用比色法测定仔鼠肝组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量,用显微镜观察肝组织结构,用免疫组织化学法检测肝组织c-Fos蛋白的表达。结果麻黄素组仔鼠10d、15d时肝组织SOD、CAT的活性低于对照组,MDA含量高于对照组(P<0.05或P<0.01),肝组织有不同程度的损伤,肝组织c-Fos蛋白表达强度高于对照组(P<0.05或P<0.01);中药组仔鼠肝组织SOD、CAT活性升高,MDA含量降低(P<0.05或P<0.01),肝组织损伤明显减轻,肝组织c-Fos蛋白的阳性表达强度减弱(P<0.05或P<0.01)。结论麻黄素对肝组织有明显的损伤效应,中药复方液可提高细胞抗氧化活性,具有抗炎、抗氧化和保护肝细胞等作用,能有效减轻麻黄素对肝组织的损伤。
Objective To investigate the effect of compound Chinese herbal medicine on ephedrine induced injury. Methods The mice were injected intraperitoneally with increasing concentrations of 2.0g / L, 3.0g / L and 4.0g / L of ephedrine solution. The rats were intragastrically treated with ephedrine for 1h, and the concentrations were 20.0g / L, 30.0g / L And 40.0g / L of traditional Chinese medicine compound solution. The activities of superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) in the liver of offsprings were determined by colorimetry at 5, 10 and 15 days after gavage. The liver tissue structure was detected by immunohistochemistry c-Fos protein expression. Results The activities of SOD and CAT in liver tissue of ephedrine group were lower than those of the control group on the 10th and 15th day. The content of MDA in the ephedrine group was higher than that of the control group (P <0.05 or P <0.01) (P <0.05 or P <0.01). The activities of SOD and CAT in the liver of the experimental group were increased, the content of MDA was decreased (P <0.05 or P <0.01), and the damage of hepatic tissue was relieved. The positive expression of c-Fos protein in liver tissue was weakened (P <0.05 or P <0.01). Conclusion Ephedrine has obvious damaging effect on liver tissue. Traditional Chinese medicine compound liquid can improve cell anti-oxidant activity, anti-inflammatory, anti-oxidant and protect hepatocytes, which can effectively reduce the damage of ephedrine to liver tissue.