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目的:观察加味连理汤对溃疡性结肠炎小鼠结肠黏膜组织中干扰素(IFN)-γ和白细胞介素(IL)-4的影响。方法:采用葡聚糖硫酸钠(DSS)饮用制备小鼠溃疡性结肠炎模型。将60只小鼠随机分为正常组、模型组、中药组、西药组各15只,光镜观察结肠黏膜组织损伤评分,采用ELISA法测定结肠黏膜中的IFN-γ、IL-4含量。结果:与模型组比较,中药组结肠黏膜组织损伤评分降低(P<0.01);结肠黏膜IFN-γ浓度下降(P<0.01),IL-4水平升高(P<0.01),IFN-γ/IL-4比值下降(P<0.01)。结论:加味连理汤对溃疡性结肠炎有显著治疗作用,机制与其抑制IFN-γ的表达和提高IL-4的表达有关。
Objective: To observe the effect of Jiaweilianli Decoction on interferon-γ and interleukin-4 in colonic mucosa of mice with ulcerative colitis. Methods: The mouse ulcerative colitis model was prepared by drinking dextran sodium sulfate (DSS). Sixty mice were randomly divided into normal group, model group, traditional Chinese medicine group and western medicine group, 15 rats in each group. Colon mucosal injury score was observed by light microscope. The contents of IFN-γ and IL-4 in colonic mucosa were determined by ELISA. Results: Compared with the model group, the colon mucosal injury scores of the TCM group decreased (P <0.01), the concentration of IFN-γ in the colonic mucosa decreased (P <0.01), the level of IL-4 increased IL-4 ratio decreased (P <0.01). Conclusion: Jiaweilianli Decoction has a significant therapeutic effect on ulcerative colitis, and its mechanism is related to inhibiting the expression of IFN-γ and increasing the expression of IL-4.