Objective: To investigate the influence of acupuncture at the points of Foot-Yangming Meridian on intracellular concentration of Ca2, called the 2nd messenger of gastric smooth muscles. Methods: 45rabbits were randomly divided into the following 5 groups: a normal saline group, a model group treated with atropine, an acupuncture group treated by needling the points of Foot-Yangming Meridian, an acupuncture group treated by needling the points of Foot-Shaoyang Meridian, an acupuncture group treated by needling the points of Foot-Taiyang Meridian, i.e. 9 rabbits in each group. After treatment, the smooth muscles of the gastric antrum were taken to make the suspension containing alive single muscular cells, and the intracellular calcium concentration ([Ca2+]i) was determined by a spectrofluorometer.Results: The concentration of [Ca2+]i in the group of Foot-Yangming Meridian was obviously higher than that of the atropine group (P＜0.01), but with no significant differences found among all the other groups (P＞0.05). Conclusion: The influence of acupuncture at the points of Foot-Yangming Meridian on gastric movement is related to the release of intracellular Ca2+ in the gastric smooth muscles.