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目的观察慢性应激对C57BL/6J小鼠行为和血液中血脂的影响。方法将20~25 g雄性C57BL/6J小鼠分成应激组及相应的对照组。应激组小鼠独笼饲养,将6个日相和6个夜相刺激及1个全天刺激随机安排到1周内,每周刺激顺序随机重新组合,连续8周。对照组动物则每5~6只小鼠合笼饲养,自由给水,整个实验过程中不接受任何刺激。2组动物均于刺激后的1、2、4和8周经内眦取血,用于血脂的检测。结果行为学实验结果显示:除第8周外,刺激后各时间点模型组小鼠体重均明显低于对照组(P<0.05)。空腹胆固醇及甘油三酯检查结果显示:与对照组相比较,模型组小鼠自刺激后的第2、4、8周显著增加(P<0.01),相反甘油三酯则在个时间点均显著降低(P<0.05)。结论复合式慢性应激是一种建立应激动物模型的有效方式;应激形成过程中动物体内胆固醇的增加提示长期的应激很可能对心血管系统造成一定危害。
Objective To observe the effects of chronic stress on behavior and blood lipids in C57BL / 6J mice. Methods Male C57BL / 6J mice (20-25 g) were divided into stress group and corresponding control group. The mice in the stress group were housed alone. Six day phase and six night phase stimulation and one all-day stimulation were randomly assigned to one week. The stimulus groups were randomly re-combined for 8 weeks. The control group of animals every 5 to 6 mice cage feeding, free water, the entire experiment does not accept any stimulation. Animals in both groups were bled by internal fossa at 1, 2, 4 and 8 weeks after stimulation for the detection of blood lipids. Results The results of behavioral experiments showed that except for the 8th week, the body weight of the model group at each time point after stimulation was significantly lower than that of the control group (P <0.05). The results of fasting cholesterol and triglyceride examination showed that compared with the control group, the mice in the model group increased significantly (P <0.01) at the 2nd, 4th, and 8th week after the stimulation, whereas the triglycerides at the 2nd, Decreased (P <0.05). Conclusions Compound chronic stress is an effective way to establish animal models of stress. The increase of cholesterol in animals during the process of stress suggests that long-term stress is likely to cause some damage to the cardiovascular system.