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目的建立大鼠重度失血性休克模型和大鼠失血性休克-肠系膜微循环模型,用以探讨携氧代血浆的扩容和携氧功能。方法将20只SD大鼠失血至全身血容量的60%,造成重度失血性休克大鼠模型后,随机均分为6%羟乙基淀粉(HES)组和携氧代血浆组做相应样品的等容量输注复苏。分别在造模前、休克、输液末以及输液后1 h观测大鼠平均动脉压(MAP)、股动脉取血测定血气,检测组织氧分压(PtO_2)。另取20只大鼠建立失血性休克-肠系膜微循环模型,观察、记录大鼠造模前基础值、休克、输液末以及输液后1 h微血管直径的变化和血流状态。结果 HES组与携氧代血浆组比较,输液末微静脉直径(μm)与微动脉直径(μm)分别为:31.23±4.96 vs 31.86±5.43、19.46±1.47 vs19.40±2.10(P>0.05),输液后血流状态均得到恢复;输液后1 h的MAP(mm Hg)为:90.70±3.83 vs 98.90±9.18(P<0.05);组织氧分压(mm Hg)为:输液末7.03±1.47 vs 15.32±2.34(P<0.05),输液后1 h 5.53±2.39 vs 8.90±2.01(P<0.05);2组大鼠血气指标在各时间点相近(P>0.05)。结论携氧代血浆能够改善大鼠失血性休克所致的微循环障碍,具有与HES相似的血管扩容作用,并且在大鼠休克复苏初期恢复组织氧分压的效果优于HES。
Objective To establish a rat model of severe hemorrhagic shock and hemorrhagic shock - mesenteric microcirculation model in rats to investigate the capacity expansion and oxygen carrying capacity of oxygen-carrying plasma. Methods 20 SD rats were sacrificed to 60% of systemic blood volume. After severe hemorrhagic shock, the rats were randomly divided into 6% hydroxyethyl starch (HES) group and oxygenated plasma group Equivalent volume infusion resuscitation. The mean arterial pressure (MAP), the femoral artery blood and the partial pressure of oxygen (PtO2) were measured before modeling, shock, infusion and 1 h after infusion respectively. Another 20 rats were established hemorrhagic shock - mesenteric microcirculation model was observed and recorded before basal modeling rats, shock, infusion end and 1 h after infusion of microvascular diameter changes and blood flow state. Results Compared with oxygen-carrying plasma group, the diameter of intravenous injection (μ m) and the diameter of arterioles (μm) in HES group were 31.23 ± 4.96 vs 31.86 ± 5.43, 19.46 ± 1.47 vs 19.40 ± 2.10 (P> 0.05) (P <0.05); MAP (mm Hg) at 1 h after transfusion was 90.70 ± 3.83 vs 98.90 ± 9.18 (P <0.05); tissue oxygen pressure (mm Hg) was 7.03 ± 1.47 vs 15.32 ± 2.34 (P <0.05). After 1 hour of infusion, 5.53 ± 2.39 vs 8.90 ± 2.01 (P <0.05). The blood gas indexes of the two groups were similar at all time points (P> 0.05). CONCLUSION: Oxo-substituted plasma can improve the microcirculation disorder induced by hemorrhagic shock in rats and has similar vasodilator effect to HES, and its effect in restoring tissue oxygen partial pressure is better than HES in the early stage of shock resuscitation.