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目的研究胃乐散对大鼠胃溃疡黏膜碳酸氢盐分泌的影响,并探讨其作用机制。方法 SD大鼠48只,随机分为正常对照组,模型组,胃乐散低剂量组[0.075g/(mL·kg)]、中剂量组[0.150g/(mL·kg)]、高剂量组[0.300g/(mL·kg)]和雷尼替丁组[0.030g/(mL·kg)],每组8只。采用冰醋酸烧灼法制备大鼠胃溃疡模型,制模后第2天,各用药组开始给药,连续用药后第14天处死大鼠,取溃疡部位组织提取蛋白,用免疫印迹法(Western blot)检测组织Cl-/HCO-3离子交换体solute carrier26A3(SLC26A3)及solute carrier26A6(SLC26A6)的含量;将大鼠胃溃疡及周围组织切片,用免疫荧光法观察囊性纤维化跨膜转运调节因子(cystic fibrosis transmembrane conductance regulator,CFTR)的变化。结果与模型组比较,用药后胃乐散高剂量组及雷尼替丁组大鼠胃黏膜中SLC26A3的表达增加,差异有统计学意义(P<0.05);雷尼替丁组,胃乐散中、高剂量组大鼠胃黏膜中SLC26A6的表达均增加,差异有统计学意义(P<0.05,P<0.01);胃乐散中、高剂量组大鼠胃溃疡黏膜的CFTR表达也明显增加,差异有显著统计学意义(P<0.01)。结论胃乐散能提高大鼠胃溃疡黏膜SLC26A3、SLC26A6和CFTR的表达,增加碳酸氢盐的分泌,保护胃黏膜。
Objective To study the effect of Weilean on gastric mucosal bicarbonate secretion in rats with gastric ulcer and to explore its mechanism. Methods Forty-eight SD rats were randomly divided into normal control group, model group, Weilean low-dose group [0.075g / (mL · kg)] and middle dose group [0.150g / (mL · kg) Group [0.300 g / (mL · kg)] and ranitidine group [0.030 g / (mL · kg)], 8 in each group. The rat model of gastric ulcer was prepared by cauterization with acetic acid. On the second day after the model was established, each drug group began to be administered, and the rats were killed on the 14th day after the continuous administration. The protein was extracted from the ulcer tissue and detected by Western blot ) Were used to detect the contents of solute carrier26A3 (SLC26A3) and solute carrier26A6 (SLC26A6) in tissue Cl- / HCO-3 ion exchangers. The gastric ulcer and surrounding tissue sections of rats were examined by immunofluorescence to observe the expression of cystic fibrosis transmembrane transporter (cystic fibrosis transmembrane conductance regulator, CFTR) changes. Results Compared with model group, the expression of SLC26A3 in gastric mucosa of high-dose Weilenshan Granules group and ranitidine group was significantly increased after treatment (P <0.05), while ranitidine group and Weile San The expression of SLC26A6 in gastric mucosa of middle and high dose groups were increased, the difference was statistically significant (P <0.05, P <0.01); the CFTR expression of gastric ulcer mucosa was also significantly increased in Weilean medium and high dose groups , The difference was statistically significant (P <0.01). Conclusion Weileosan can improve the expression of SLC26A3, SLC26A6 and CFTR in gastric ulcer mucosa, increase bicarbonate secretion and protect gastric mucosa.